Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation
نویسندگان
چکیده
منابع مشابه
Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation.
Magnetic-activated cell sorting (MACS) using paramagnetic annexin V-conjugated microbeads eliminates spermatozoa with externalized phosphatidylserine, which is considered one of the features of apoptosis. The objective of this study was to evaluate sperm recovery following the use of MACS as a sperm preparation technique. Mature spermatozoa were separated and divided into two fractions: the fir...
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Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...
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Sperm DNA integrity is an important paternal factor that has been shown to influence fertilization rates, embryo development and pregnancy rates. To reduces the number of apoptotic spermatozoa many technics are used before ICSI treatment ( e.g. using of antioxidants, testicular spermatozoa). Recently, the use of annexin V microbeads, as a non-invasive method to reduce high levels of sperm apopt...
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Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort dama...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2006
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)61437-x